Résultats pour gfp mice

gfp mice
007788 STOCK TgThy1-EGFPMJrs/J.
A 27 SNP single nucleotide polymorphism panel analysis performed by The Jackson Lpboratory on the rederived mice revealed that this strain is on a mixed background six markers are segregating: 4 as CBA and 2 as neither C57BL/6 nor CBA July 2009. Expressed Gene GFP, Green Fluorescent Protein.,
GFP Antibody Monoclonal, 3E6.
GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer FRET experiments.
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Anti-GFP antibody 9F9.F9 ab1218 Abcam.
Can be paired for Sandwich ELISA with Goat polyclonal to GFP Biotin ab6658. ab1218 can be used to detect GFP by ELISA sandwich or capture for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP as a capture antibody.
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Strain description These transgenic mice express a fusion cDNA encoding enhanced GFP jointed at its C-terminus to rat microtubule-associated protein 1 light chain 3 beta under the control of the CAG promoter. The occurrence of autophagy in mouse tissues can be monitored by the GFP.
Does anyone have experience in visualizing eGFP mice?
I also used something like this apparatus see link, it's' portable. I presume your problem is more related to the intensity of your lump or your GFP expression levels and not wave length. If you just need an indication of positive GFP expressing mice and all else fails you can also do RTPCR from your mice with any tissue sample.
003291 C57BL/6-TgCAG-EGFP1Osb/J.
This transgenic mouse line, with an enhanced" GFP EGFP cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer, have widespread EGFP fluorescence, with the exception of erythrocytes and hair. Note that the donating investigator reports that mice homozygous for this transgene die within the first two weeks following birth.
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Frederick National Lab: LASP Mouse Models Available Strain Details.
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Expression Analysis of CB2-GFP BAC Transgenic Mice.
Therefore, we have generated BAC transgenic GFP reporter mice CB2-GFPTg to trace CB2 expression in vitro and in situ. Those mice express GFP under the CB2 promoter and display GFP expression paralleling CB2 expression on the transcript level in spleen, thymus and brain tissue.
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Green Fluorescent Protein: Properties, Applications and Protocols Google Livres.
Acad Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chale chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics efcient elegans embryos emission energy transfer excitation expressing GFP lter rst ow Fluorescent uorophore FRET function fusion protein gene expression genetic GFP expression GFP uorescence GFP fusion proteins GFP-like proteins green uorescent protein Heim identied imaging interactions jellysh living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy microtubules molecular molecules Morin mutants Natl neurons nuclear peptide photobleaching plasmid Prasher Proc quantum yield RCFPs receptor recombinant red uorescent protein Renilla luciferase residues screening sequence Shimomura signal specic spectral studies subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrash.

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