Résultats pour gfp sequence

gfp sequence
The Green Boxer Fluorescent Lab. Protein.
259, 267 We aim to use this highly controlled system to better understand the biophysics of split GFP reconstitution in vitro in order to inform experiments using split GFP systems in vivo. We are also interested in using our synthetic methods for producing fluorescent proteins with novel photophysical properties, as well as better understanding the photochemistry of GFP. In addition to protein engineering, we use ultrafast fluorescence and a host of other analytical methods to characterize these novel proteins. Synthetic" Control of Green Fluorescent Protein, Kevin P.
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Aequorea victoria green-fluorescent protein GFP mRNA, complete cds Nucleotide NCBI.
PubMed articles cited by Nucleotide sequence record. Taxonomy sequences associated with Nucleotide record. Full text in PMC. Free full text articles in PMC. LinkOut to external resources. Order GFP cDNA clone/Protein/Antibody/RNAi OriGene. Order GFP cDNA clone/Protein/Antibody/RNAi. Clear Turn Off Turn On.
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Green Fluorescent Protein: Properties, Applications and Protocols Google Livres.
Acad Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chale chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics efcient elegans embryos emission energy transfer excitation expressing GFP lter rst ow Fluorescent uorophore FRET function fusion protein gene expression genetic GFP expression GFP uorescence GFP fusion proteins GFP-like proteins green uorescent protein Heim identied imaging interactions jellysh living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy microtubules molecular molecules Morin mutants Natl neurons nuclear peptide photobleaching plasmid Prasher Proc quantum yield RCFPs receptor recombinant red uorescent protein Renilla luciferase residues screening sequence Shimomura signal specic spectral studies subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrash.
Green Fluorescent Protein The Embryo Project Encyclopedia. Green Fluorescent Protein.
Chalfie's' team obtained the cDNA of the gene Gfp from Prasher and inserted only the coding sequence of Gfp gene first in the bacterium Escherichia Coli, and then in C. Chalfie and his team found that Gfp gene produced GFP without added enzymes or substrates in both organisms.
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Common Cloning DNA Sequences.
Below is a list of several common sequences used in molecular biology, including sequences to plasmid features T7, SP6, CMV, etc, resistance genes AMP, Kan, etc, and protein tags GFP, myc, FLAG. These sequences come standard for analysis and use with the GeneCoder software package. Please note these common DNA sequences are approximate different variants may exist that vary slightly from the sequences below. To go directly to the sequence, click the appropriate sequence name in the table below.
Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter.
This sequence undergoes spontaneous oxidation to form a cyclized chromophore 6. Enhanced GFP EGFP contains mutations of Ser to Thr at amino acid 65 and Phe to Leu at position 64 and is encoded by a gene with human-optimized codons 7-9.
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Green fluorescent protein Wikipedia.
PDBsum structure summary. Green fluorescent protein. Organism Aequorea victoria. The green fluorescent protein GFP is a protein composed of 238 amino acid residues 26.9 kDa that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. 2 3 Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria, avGFP. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.
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pMXs-IRES-GFP Retroviral Expression Vector.
Nature 540 276-279. Nicotinamide phosphoribosyltransferase inhibits receptor activator of nuclear factor-kB ligand-induced osteoclast differentiation in vitro. Dendritic cell-specific transmembrane protein DC-STAMP regulates osteoclast differentiation via the Ca2/NFATc1 axis. Polarization of M2 macrophages requires Lamtor1 that integrates cytokine and amino acid signals.
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mNeonGreen: A green fluorescent protein that is not a GFP variant.
Excitation Emission wavelengths and brightness of mNeonGreen. Furthermore, mNeonGreen has an excitation maximum at 506 nm and an emission maximum at 517 nm Shaner et al, 2013. mNeonGreen is compatible with most GFP filter sets. Apparently, it is 3 times brighter than GFP when using GFP filters; optimization of filters may increase its brightness further. In addition, mNeonGreen seems to be more stable and less sensitive to laser induced bleaching than EGFP. Therefore, mNeonGreen is particular suitable for confocal and super resolution microscopy, especially when fusion proteins are investigated, which are expressed at low levels. Size and Structure of mNeonGreen.
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