Résultats pour gfp structure

 
gfp structure
 
Green fluorescent protein: structure, folding and chromophore maturation. PubMed NCBI.
Conserved Domain Search Service CD Search. PubChem Structure Search. SNP Submission Tool. Vector Alignment Search Tool VAST. All Data Software Resources. Conserved Domain Database CDD. Conserved Domain Search Service CD Search. Structure Molecular Modeling Database. Vector Alignment Search Tool VAST.
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GFP-tagging in Fluorescence Microscopy.
The structure of GFP includes the primary structure of a chain of 238 amino acids, and a secondary structure of hydrogen-bonded helices and pleated sheets and a tertiary barrel shaped structure capped with helices. Within the center is the chromophore, an amino acid section responsible for the light emission.
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Introduction aux techniques utilisées en biochimie.
R Sikorsi, R Peters 1997 Lighting" Rods, Science 2781485-6. commentaires sur des applications potentielles de protéines chimériques à base de GFP. M Ormö et al 1996 Crystal structure of the Aequorea victoria green fluorescent protein, Science 2731392-5. structure de la GFP.
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Green Fluorescent Protein: Properties, Applications and Protocols Google Livres.
4 THE THREEDIMENSIONAL STRUCTURE OF GREEN FLUORESCENT PROTEIN AND ITS IMPLICATIONS FOR FUNCTION AND DESIGN. 5 MOLECULAR BIOLOGY AND MUTATION OF GREEN FLUORESCENT PROTEIN. 6 DISCOVERY AND PROPERTIES OF GFPLIKE PROTEINS FROM NONBIOLUMINESCENT ANTHOZOA. 7 EVOLUTION OF FUNCTION AND COLOR IN GFPLIKE PROTEINS. 8 THE USES OF GREEN FLUORESCENT PROTEIN IN PROKARYOTES. 11 GREEN FLUORESCENT PROTEIN APPLICATIONS IN DROSOPHILA. 12 THE USES OF GREEN FLUORESCENT PROTEIN IN PLANTS. 13 USES OF GFP IN TRANSGENIC VERTEBRATES.
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PDB-101: Molecule of the Month: Green Fluorescent Protein GFP.
To explore this structure in more detail, click on the image for an interactive JSmol. Topics for Further Discussion. You can explore an annotation of GFP and its family relationships at Interpro in their feature The Green Fluorescent Protein. Related PDB-101 Resources.
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Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222.
In the case of wt GFP Ser65, Tyr66 and Gly67 main-chain atoms cyclise after the protein folds to form, in the presence of O 2, the highly conjugated planar p hydroxybenzylideneimidazolinone chromophore 6. The crystal structure of wild-type GFP wt GFP 7, 8 revealed that the chromophore is packed within the core of the GFP barrel structure protecting it from quenching through water dipoles, paramagnetic oxygen or cis-trans isomerisation.
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GFP.
The barrel structure helps to protect the chromophore from quenching through water dipoles, paramagnetic oxygen, or cis-trans isomerization 3. This gives wt-GFP the ability to fluoresce at varying pHs and redox potentials and resist photobleaching 1. The chromophore of wt-GFP is a p hydroxybenzylideneimidazolinone formed from residues Ser-65, Tyr-66, and Gly-67 1. The chromophore is formed via a three-step autocatalytic process that occurs after GFP folds into its native conformation Image 1.
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Protéine fluorescente verte Wikipédia.
Limage montre la sculpture en acier inoxydable aux Friday Harbor Laboratories de San Juan Island Washington, là où la GFP a été découverte. Julian Voss-Andreae, un artiste dorigine allemande spécialisé dans les sculptures de protéines 9, a réalisé des sculptures sinspirant de la structure de la GFP, notamment la sculpture de 170, m de hauteur intitulée Green Fluorescent Protein 2004 10 et la sculpture Steel Jellyfish 2006 de 140, m de hauteur.
La GFP: La GFP.
B: Structure tridimensionnelle de la GFP. L'hélice' contient dans sa séquence en acides aminés les 3 acides aminés responsables de la fluorescence de la protéine, ils forment le groupe chromophore, dont nous approfondirons le mécanisme dans la seconde partie. Lexique de" La GFP."

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