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gfp structure
GFP-tagging in Fluorescence Microscopy.
The structure of GFP includes the primary structure of a chain of 238 amino acids, and a secondary structure of hydrogen-bonded helices and pleated sheets and a tertiary barrel shaped structure capped with helices. Within the center is the chromophore, an amino acid section responsible for the light emission.
B: Structure tridimensionnelle de la GFP. L'hélice' contient dans sa séquence en acides aminés les 3 acides aminés responsables de la fluorescence de la protéine, ils forment le groupe chromophore, dont nous approfondirons le mécanisme dans la seconde partie. Lexique de" La GFP."
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Green fluorescent protein: structure, folding and chromophore maturation. PubMed NCBI.
BLAST Basic Local Alignment Search Tool. Conserved Domain Search Service CD Search. PubChem Structure Search. SNP Submission Tool. Vector Alignment Search Tool VAST. All Data Software Resources. Conserved Domain Database CDD. Conserved Domain Search Service CD Search. Structure Molecular Modeling Database.
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EGFP has a molecular weight of 26897.43, Da and a pI of 5.58 2. EGFP exists as a monomer and also has the same secondary structures as wt-GFP because the mutations occur within the core 3 10 helix and chromophore and do not affect overall protein structure.
PDB-101: Molecule of the Month: Green Fluorescent Protein GFP.
Notice how the glycine and the threonine have formed a new bond, creating an unusual five-membered ring. To explore this structure in more detail, click on the image for an interactive JSmol. Topics for Further Discussion. You can explore an annotation of GFP and its family relationships at Interpro in their feature The Green Fluorescent Protein.
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Green Fluorescent Protein The Embryo Project Encyclopedia. Green Fluorescent Protein.
Gfp refers to the gene that produces green fluorescent protein. Using DNA recombinant technology, scientists combine the Gfp gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell.
GFP Green fluorescent protein Aequorea victoria Jellyfish GFP gene protein.
Cited for: NUCLEOTIDE SEQUENCE MRNA, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY. The" structural basis for spectral variations in green fluorescent protein" Palm G.J., 4361-3651997: PubMed Europe PMC Abstract. Cited for: X-RAY CRYSTALLOGRAPHY 2.00 ANGSTROMS, MUTAGENESIS OF PHE-64; TYR-66; SER-72; ASN-149; MET-153 AND ILE-167, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY. Structural" basis of spectral shifts in the yellow-emission variants of green fluorescent protein" Wachter R.M., Structure 61267-12771998: PubMed Europe PMC Abstract. Cited for: X-RAY CRYSTALLOGRAPHY 2.5 ANGSTROMS OF YELLOW EMISSION MUTANT TYR-203/GLY-65/LEU-68/ALA-72, MUTAGENESIS OF SER-65; VAL-68; SER-72 AND THR-203, BIOPHYSICOCHEMICAL PROPERTIES, BIOTECHNOLOGY, SUBUNIT. Structural" and spectral response of green fluorescent protein variants to changes in pH" Elsliger M.-A., Biochemistry 385296-53011999: PubMed Europe PMC Abstract. Cited for: X-RAY CRYSTALLOGRAPHY 2.0 ANGSTROMS, BIOPHYSICOCHEMICAL PROPERTIES. X-ray" structure of Cerulean GFP: a tryptophan-based chromophore useful for fluorescence lifetime imaging" Malo G.D.,
Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222.
In the case of wt GFP Ser65, Tyr66 and Gly67 main-chain atoms cyclise after the protein folds to form, in the presence of O 2, the highly conjugated planar p hydroxybenzylideneimidazolinone chromophore 6. The crystal structure of wild-type GFP wt GFP 7, 8 revealed that the chromophore is packed within the core of the GFP barrel structure protecting it from quenching through water dipoles, paramagnetic oxygen or cis-trans isomerisation.
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L'instantané' du mois: GFP.
Et pour cause. La GFP a la forme d'un' tonneau presque parfait! Au-delà de sa régularité fort singulière, la structure très compacte de la protéine offre l'avantage' de protéger son cur fluorescent de tout dommage chimique. Comment la GFP acquiert-elle l'extraordinaire' propriété d'émettre' de la fluorescence?
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