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gfp gene
 
GFP Sequence and Map.
Your time is valuable! Basic Cloning Vectors CRISPR Plasmids Gateway Cloning Vectors TA and GC Cloning Vectors Fluorescent Protein Genes Plasmids GFP Luciferase Vectors Mammalian Expression Vectors Viral Expression Packaging Vectors I.M.A.G.E. Consortium Plasmids Yeast Plasmids Plant Vectors Insect Cell Vectors Lucigen Vectors pET Duet Vectors Novagen pGEX Vectors GE Healthcare Qiagen Vectors Structural Genomics Vectors. Aequoria victoria green fluorescent protein. To obtain this DNA and protein sequence with restriction sites, please download.
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Green Green Fluorescent Fluorescent Protein Protein GFP GFP Thermo Thermo Fisher Fisher Scientific Scientific BE. US.
Cells expressing GFP show dual labeling of both GFP green fluorescence and the anti-GFP antibody red fluorescence. In this overlay of fluorescence and differential interference contrast DIC micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI stains the nuclei with a light-blue fluorescence.
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Use green fluorescent protein to tag expression of genes: DNA from the Beginning.
Both the pX plasmid and the DNA with the GFP gene should be cut with the enzymes PvuI and SalI. The complementary ends made by the enzymes are such that the GFP gene can only be inserted in the forward orientation.
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GFPLC3B: vector to visualize autophagosome formation in real time.
pSELECT-GFP-LC3 are mammalian expression vectors containing the human or murine LC3B gene fused at its 5 end to the green fluorescent protein GFP gene. The same plasmid is available with the GFP gene alone as a control. This control plasmid is called pSELECT-NGFP-zeo.
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Understanding Genetics.
The first step in getting the GFP gene was purifying the GFP protein. The genetic code that creates all the complexity around us has only 64 words with 20 or so definitions. After is was cloned the first time, getting the GFP gene was easy.
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PDF EXPRESSION IN VIVO DU GÈNE GFP SOUS LA FORME D'UNE' PROTÉINE DE FUSION.
A complementary DNA for the Aequorea victoria green fluorescent protein GFP produces a fluorescent product when expressed in prokaryotic Escherichia coli or eukaryotic Caenorhabditis elegans cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
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Green Fluorescent Protein Molecule of the Month January 2010 HTML-only version.
So why is this important? Well this means that organisms, other than those in which GFP naturally occurs, can be genetically engineered to have a gene that produces GFP and it will still work without multiple other genes being implanted 22.
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Green Fluorescent Protein: A Molecular Microscope.
The manufacture of proteins using the instructions from the gene is called protein expression. Right: The GFP gene is inserted at the end of the protein gene, but before the stop codon. The resultant GFP-fusion protein shown in the right image is fluorescent, and can functionally replace the original protein.
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GFP Applications.
The GFP gene is fused with the gene encoding the protein of interest and expressed in the cell. The incorporation of GFP can be done at either the amino or the carboxyl terminus of a protein. The fused protein maintains its normal function along with acquired fluorescent property through GFP expressing gene.
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