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Aequorea victoria green-fluorescent protein GFP mRNA, complete cds Nucleotide NCBI.
PubMed articles cited by Nucleotide sequence record. Taxonomy sequences associated with Nucleotide record. Full text in PMC. Free full text articles in PMC. LinkOut to external resources. Order GFP cDNA clone/Protein/Antibody/RNAi OriGene. Order GFP cDNA clone/Protein/Antibody/RNAi. Clear Turn Off Turn On.
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Green Fluorescent Protein: Properties, Applications and Protocols Google Livres.
Acad Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chale chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics efcient elegans embryos emission energy transfer excitation expressing GFP lter rst ow Fluorescent uorophore FRET function fusion protein gene expression genetic GFP expression GFP uorescence GFP fusion proteins GFP-like proteins green uorescent protein Heim identied imaging interactions jellysh living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy microtubules molecular molecules Morin mutants Natl neurons nuclear peptide photobleaching plasmid Prasher Proc quantum yield RCFPs receptor recombinant red uorescent protein Renilla luciferase residues screening sequence Shimomura signal specic spectral studies subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrash.
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Green Fluorescent Protein The Embryo Project Encyclopedia. Green Fluorescent Protein.
Chalfie's' team obtained the cDNA of the gene Gfp from Prasher and inserted only the coding sequence of Gfp gene first in the bacterium Escherichia Coli, and then in C. Chalfie and his team found that Gfp gene produced GFP without added enzymes or substrates in both organisms.
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Common Cloning DNA Sequences.
Below is a list of several common sequences used in molecular biology, including sequences to plasmid features T7, SP6, CMV, etc, resistance genes AMP, Kan, etc, and protein tags GFP, myc, FLAG. These sequences come standard for analysis and use with the GeneCoder software package. Please note these common DNA sequences are approximate different variants may exist that vary slightly from the sequences below. To go directly to the sequence, click the appropriate sequence name in the table below.
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PartBBa: E0040 parts.igem.org.
Improved by BNU-China 2019. We design a device for increasing the degradation rate of green fluorescent protein GFP by adding a 16-amino-acid-long tag replication protein A RepA at the N-terminal, therefore the green fluorescence will degrade sooner when expression ends. Please view BBa_K3036006 for more details. Protein data table for BioBrick BBa_E0040 automatically created by the BioBrick-AutoAnnotator version 1.0. Nucleotide sequence in RFC 10: underlined part encodes the protein.
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Protéine fluorescente verte Wikipédia.
Clan Pfam CL0069. La protéine fluorescente verte souvent abrégé GFP, de l anglais Green Fluorescent Protein est une protéine ayant la propriété d'émettre' une fluorescence de couleur verte. Issue d'une' méduse Aequorea victoria, cette protéine est intrinsèquement fluorescente sous l'action' d'une' enzyme, l'aequoréine, une luciférase qui agit en présence de calcium. Son gène peut être fusionné in-vitro au gène d'une' protéine que l'on' souhaite étudier.
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GFPSpark.
Gene Name Synonym: GFP. Protein Construction A DNA sequence encoding the Aequorea Victoria GFP AAB65663 Met1-Leu238, except for the point mutations of intellectual property F64L, S65T, R80K, S99F, T153M, 167v, H231L was expressed, with a polyhistide tag at the C-terminus Patent 201410599023.9.
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mNeonGreen: A green fluorescent protein that is not a GFP variant.
Hence, mNeonGreen is evolutionarily distant from jellyfish-derived fluorescent proteins. At sequence level, mNeonGreen shares just 20-25% sequence identity with common GFP derivatives.: Comparison of EGFP1 and mNeonGreen2 amino acid sequences. The mNeonGreen DNA sequence can be found here: https//www.ncbi.nlm.nih.gov/nuccore/KC295282.1: or https//www.ebi.ac.uk/ena/data/view/KC295282.:
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Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter.
This sequence undergoes spontaneous oxidation to form a cyclized chromophore 6. Enhanced GFP EGFP contains mutations of Ser to Thr at amino acid 65 and Phe to Leu at position 64 and is encoded by a gene with human-optimized codons 7-9.
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