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gfp spectrum
Excitation and Emission of Green Fluorescent Proteins February 20, 2001.
As demonstrated in Figure 2, it is critical that the appropriate filter set be utilized for the GFP variant being measured. Using the fluorescein" filter set 485/20 excitation, 530/25 emission when quantitating wtGFP results in a 20% loss of signal, when compared to the recommended 400/30 excitation, 508/20 emission filter set Figure 2B.
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Green fluorescent protein Wikipedia.
Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield QY of GFP is 0.79. The GFP from the sea pansy Renilla reniformis has a single major excitation peak at 498 nm.
Circular dichroism spectroscopy of fluorescent proteins. PubMed NCBI.
The CD spectrum of DsRed is anomalous in the sense that the major CD band has a clear excitonic character. Far-UV CD spectra of GFP confirmed the presence of the high beta-sheet content of the polypeptide chain in the three-dimensional structure.
Green Fluorescent Protein GFP Thermo Fisher Scientific US.
Cells expressing GFP show dual labeling of both GFP green fluorescence and the anti-GFP antibody red fluorescence. In this overlay of fluorescence and differential interference contrast DIC micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI stains the nuclei with a light-blue fluorescence.
The enhanced green fluorescent protein as a tool for the analysis of protein dynamics and localization: local fluorescence study at the single-mole. PubMed NCBI.
Here we present a detailed study of the fluorescence properties of the Phe-64-Leu, Ser-65-Thr mutant down to the single molecule level in order to assess its use in quantitative fluorescence microscopy and single-protein trafficking. This enhanced GFP EGFP is being used extensively as it offers higher-intensity emission after blue-light excitation with respect to wild-type GFP.
Spectrum GFP emerald GFP AAT Bioquest.
For technical assistance on using this web application, please contact You are currently viewing the spectrum for GFP emerald GFP. GFP emerald GFP has an excitation maximum at 489 nm. GFP emerald GFP has an emission maximum at 508 nm.
Spectra Viewer Chroma Technology Corp.
All transmission and blocking OD data are actual, measured spectra of representative production lots. Spectra varies slightly from lot to lot. Optical density values in excess of 6 may appear noisy because such evaluations push the resolution limit of low light level measurements.

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