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PDB-101: Molecule of the Month: Green Fluorescent Protein GFP.
It is easy to find out where GFP is at any given time: you just have to shine ultraviolet light, and any GFP will glow bright green. So here is the trick: you attach the GFP to any object that you are interested in watching.
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Structural Dynamics of Green Fluorescent Protein Alone and Fused with a Single Chain Fv Protein.
However, the fluorescence anisotropy decays more rapidly than can be expected for a fusion product, which is about twice the size of a single GFP molecule Fig. For globular proteins the rotational correlation time is proportional to the molecular mass.
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GFP Antibody B-2 SCBT Santa Cruz Biotechnology.
Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
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Green Fluorescent Protein: Properties, Applications and Protocols Google Livres.
Acad Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chale chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics efcient elegans embryos emission energy transfer excitation expressing GFP lter rst ow Fluorescent uorophore FRET function fusion protein gene expression genetic GFP expression GFP uorescence GFP fusion proteins GFP-like proteins green uorescent protein Heim identied imaging interactions jellysh living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy microtubules molecular molecules Morin mutants Natl neurons nuclear peptide photobleaching plasmid Prasher Proc quantum yield RCFPs receptor recombinant red uorescent protein Renilla luciferase residues screening sequence Shimomura signal specic spectral studies subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrash.
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The Size of GFP Science Buddies Blog.
In Sizing" It Up! How Scientists Separate Proteins, a science fair project idea authored by a teacher-student team from Tracy High School in San Joaquin county, CA, students use a Size-exclusion Chromatography Kit from Bio-Rad, sponsor of Science Buddies biotechnology projects and resources, to explore size-exclusion chromatography as they create mixtures and break them down again to determine the size of GFP, a protein which glows green when exposed to blue light.
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GFP Products: RD Systems.
GFP Alexa Fluor 594-conjugated Antibody. Show Size Price. GFP Alexa Fluor 405-conjugated Antibody. Show Size Price. GFP Alexa Fluor 488-conjugated Antibody. Show Size Price. GFP Alexa Fluor 700-conjugated Antibody. Show Size Price. GFP Alexa Fluor 647-conjugated Antibody. Show Size Price.
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Anti-GFP Antibody Products Biocompare.com.
Supplier Page Sign In or Register to view pricing. GFP Antibody, pAb, Rabbit. Write a Review. Supplier Page Sign In or Register to view pricing. See More 2. View All 4. GFP Polyclonal Antibody: Trial Size. WB, ICC, IHC, IP, ChIP, EM.
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gfp green fluorescent protein Neisseria gonorrhoeae Gene NCBI.
Contact Help Desk. Clear Turn Off Turn On. gfp Neisseria gonorrhoeae. gfp Neisseria gonorrhoeae. Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics. Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells. Journal of Experimental Botany.
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