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gfp molecular weight
 
GFP Recombinant Aequorea victoria Protein, N-His Tag Thermo Fisher Scientific.
The purity and molecular weight of the recombinant human GFP were determined by SDS-PAGE of reduced GFP, followed by staining with Coomassie Blue" data-omni-actionView" figure" SDS-PAGE analysis of Green Fluorescent Protein GFP. Gene Aliases: GFP. Gene Symbol: GFP. Molecular Weight: 28.7 kDa.
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Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222.
The apparent molecular weight was still very close to the theoretical molecular weight calculated from the amino acid sequence 26941 Da. The elution peak was non-symmetrical, suggesting there was more than one oligomeric species present in dynamic equilibrium with the monomeric form; this is consistent with previous observations that wt GFP is largely monomeric with a weak tendency to dimerise.
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GFP Introduction.
The evolutionary relationship of Aequorea GFP and Renilla GFP is not known, since the amino acid sequence of the latter has not yet been determined. The primary structure of Aequorea GFP was deduced from the cDNA sequence 9. Aequorea GFP is a protein of 238 amino acids with a molecular weight of 27 or 30 kDa.
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Green Fluorescent Protein GFP BioVendor.
You are here: Home Products by Molecule of Interest Green Fluorescent Protein GFP. Green Fluorescent Protein GFP. Green fluorescent protein GFP isolated from jellyfish Aequorea aequorea is a 238 amino acid protein with an apparent molecular weight of about 27 30 kDa on SDS-PAGE.
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Structural Dynamics of Green Fluorescent Protein Alone and Fused with a Single Chain Fv Protein.
However, the fluorescence anisotropy decays more rapidly than can be expected for a fusion product, which is about twice the size of a single GFP molecule Fig. For globular proteins the rotational correlation time is proportional to the molecular mass.
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GFP, His-tag.
Useful as a quantitative control for GFPexpression studies using GFP reportervectors. It can also be used as a standard onprotein gels and Western immunoblots or forcalibration of fluorometers, flow cytometersand FACS machines. Chudakov DM, et al. Gerdes HH, Kaether C. 1996 Jun 24389144-7. Avoid freeze/thaw cycles. Protect from light. Data shown is lot-specific. Contact us for specific information on other lots. Qty: Add to Cart. Bulk Quote Contact Us. Screening and Profiling. Custom Cell Line Development. Molecular and Cell Biology.
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Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli.
To better understand why exported GFP was not fluorescent, we purified the MBPSS418-GFP hybrid protein to determine if it displayed fluorescence when no longer confined to the periplasmic space. To accomplish this, we took advantage of the affinity of the MBP moiety for amylose to purify MBPSS418-GFP from the periplasmic space, as well as MBPSS418-GFP from the cytoplasmic compartment. Purified proteins were analyzed by SDS-PAGE and Western blotting to assess the purity of the proteins. While both the cytoplasmic and the periplasmic versions of MBP-GFP yielded a single band of predicted molecular weight, the exported fusion also copurified with a minor fraction of MBP breakdown products data not shown.
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Fluorescent Proteins Introduction and Photo Spectral Characteristics Learn Share Leica Microsystems.
Ex: Peak excitation wavelength nm. Em: Peak emission wavelength nm. MW: Molecular weight. QY: Quantum yield. BR: Brightness; Extinction coefficient Quantum yield/1000., PS: Photostability; time to 50 % brightness sec. Wild Type GFP. Tetramer monomeric available. Monomer; faster photobleach than mPlum.
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