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gfp molecular weight
Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli.
While both the cytoplasmic and the periplasmic versions of MBP-GFP yielded a single band of predicted molecular weight, the exported fusion also copurified with a minor fraction of MBP breakdown products data not shown. As shown in Fig. Fig.7, 7, illumination of the samples by UV showed that MBPSS418-GFP isolated from the cytoplasm was highly fluorescent. In contrast, an equivalent amount of MBPSS418-GFP isolated from the periplasmic space showed no detectable fluorescence, consistent with the in vivo observations. These results indicated that the lack of fluorescence in vivo cannot be attributed solely to diminished levels of the hybrid protein and that recovery of GFP from the periplasmic environment is not sufficient to immediately restore fluorescence.
GFP tag Antibody HRP-66002 Proteintech.
HRP-66002 targets HRP-conjugated GFP tag in WB, ELISA applications and shows reactivity with recombinant protein, aequorea victoria samples. recombinant protein, aequorea victoria. Host / Isotype. Mouse / IgG2a. Calculated molecular weight. Observed molecular weight. GenBank accession number. Gene ID NCBI.
GFP, His-tag.
Africa Middle East. Size: 200 g. Host Species: E. MW: 29.9 kDa. Tags: C-terminal His-tag. a.a: full length. Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant Jellyfish Aequorea victoria green fluorescent protein, full length with C-terminal His-tag, MW 29.9 kDa, expressed in an E. coli expression system. GFP absorbs light at 395 and 470 nm, emits green fluorescence at 509 nm.
EGFP: Fluorescent Protein Database.
Many pages and features of FPbase will not work properly. enhanced GFP, GFPmut1. Add to collection. Add to favorites 56. EGFP is a basic constitutively fluorescent green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. Oligomerization Organism Molecular Weight Cofactor.
Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222.
The apparent molecular weight was still very close to the theoretical molecular weight calculated from the amino acid sequence 26941 Da. The elution peak was non-symmetrical, suggesting there was more than one oligomeric species present in dynamic equilibrium with the monomeric form; this is consistent with previous observations that wt GFP is largely monomeric with a weak tendency to dimerise.
GFP Antibody B-2 SCBT Santa Cruz Biotechnology.
Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP. For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. Alexa Fluor is a trademark of Molecular Probes Inc, OR, USA.
Enhanced Green Fluorescent Protein EGFP 4999 BioVision, Inc.
Molecular Weight 32.7 kDa. Purity by SDS-PAGE 97%. Purity by 97%. Endotoxin Level 0.1 ng/g. Reconstitution Instructions Reconstitute with PBS to 1 mg/ml. Handling Centrifuge the vial prior to opening. Storage Conditions 20C. Shipping Conditions Gel Pack. USAGE For Research Use Only! Not to be used in humans. The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence.
What is the molecular weight of DNA coding green fluorescent protein, approximately, how many base pairs are there?
Warsaw University of Technology. What is the molecular weight of DNA coding green fluorescent protein, approximately, how many base pairs are there? Green Fluorescent Protein. Popular Answers 1. GFP is around 720 bp239 AA 26.9kDa. All Answers 5. GFP is around 720 bp239 AA 26.9kDa.
GFP Introduction.
The evolutionary relationship of Aequorea GFP and Renilla GFP is not known, since the amino acid sequence of the latter has not yet been determined. The primary structure of Aequorea GFP was deduced from the cDNA sequence 9. Aequorea GFP is a protein of 238 amino acids with a molecular weight of 27 or 30 kDa.

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