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gfp molecular weight
 
GFP tag Antibody HRP-66002 Proteintech.
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at 20 o C. Aliquoting is unnecessary for 20 o C storage. Calculated molecular weight.: Observed molecular weight.: GenBank accession number.: Gene ID NCBI.: CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP.
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Green Fluorescent Protein Molecule of the Month January 2010 HTML-only version.
Green Fluorescent Protein. A molecular tag that can be inserted into genes. to make animals and plants glow green. Timothy King and Paul May. University of Bristol. Molecule of the Month January 2010. Also available: JSMol version. An Introduction to GFP. GFP stands for green fluorescent protein the official name for the molecule and is, imaginatively, a protein that fluoresces green in the presence of UV light 1. It has found its use in all areas of cellular biology and advances have reached the point where it is the focus of works of art 2, such as a pet rabbit called Alba, whose fur glowed green under UV light. In November 2008, three men, Osamu Shimomura, Martin Chalfie and Roger Tsien, were awarded the Nobel Prize 3-7 in chemistry, for" the discovery and development of green fluorescent protein. So what makes this such a versatile and important molecule? A Brief Explanation of Bioluminescence and Fluorescence.
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Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli.
One possibility considered was that this breakdown product represented GFP that had been clipped free of the MBP sequences. However, this product had a molecular mass of 33 kDa, which is significantly larger than GFP. Open in a separate window. Cell fractionation of shortened MBP-GFP hybrid protein. Cells expressing MBPSS128-GFP were fractionated into inner and outer membranes as described in Materials and Methods. The immunoblot shown was decorated with anti-GFP antibody. MBPSS128-GFP migrated at a predicted molecular mass of 40 kDa and fractionated with the outer membrane. An apparent smaller-molecular-weight derivative of this protein was also found in the inner membrane fraction. Molecular weight MW standards are as shown.
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Crystal Structure of Enhanced Green Fluorescent Protein to 1.35 Å Resolution Reveals Alternative Conformations for Glu222.
The apparent molecular weight was still very close to the theoretical molecular weight calculated from the amino acid sequence 26941 Da. The elution peak was non-symmetrical, suggesting there was more than one oligomeric species present in dynamic equilibrium with the monomeric form; this is consistent with previous observations that wt GFP is largely monomeric with a weak tendency to dimerise.
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GFP Antibody B-2 SCBT Santa Cruz Biotechnology.
Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP. For Research Use Only. Not Intended for Diagnostic or Therapeutic Use. Alexa Fluor is a trademark of Molecular Probes Inc, OR, USA.
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Enhanced Green Fluorescent Protein EGFP 4999 BioVision, Inc.
Molecular Weight 32.7 kDa. Purity by SDS-PAGE 97%. Purity by 97%. Endotoxin Level 0.1 ng/g. Reconstitution Instructions Reconstitute with PBS to 1 mg/ml. Handling Centrifuge the vial prior to opening. Storage Conditions 20C. Shipping Conditions Gel Pack. USAGE For Research Use Only! Not to be used in humans. The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence.
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EGFP: Fluorescent Protein Database.
about FPbase help FAQs how to cite FPbase database design contributing API recent activity how you can help contact. advanced search BROWSE. protein table interactive chart lineages organisms spectra references SUBMIT. submit a protein submit a spectrum EXTRA. FRET protein collections microscopes. Javascript is disabled! Many pages and features of FPbase will not work properly. enhanced GFP, GFPmut1. Add to collection. Add to favorites 25. EGFP is a basic constitutively fluorescent green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. Oligomerization Organism Molecular Weight Cofactor.
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What is the molecular weight of DNA coding green fluorescent protein, approximately, how many base pairs are there?
Warsaw University of Technology. What is the molecular weight of DNA coding green fluorescent protein, approximately, how many base pairs are there? Green Fluorescent Protein. Popular Answers 1. GFP is around 720 bp239 AA 26.9kDa. All Answers 5. GFP is around 720 bp239 AA 26.9kDa.
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GFP, His-tag.
Africa Middle East. Size: 200 g. Host Species: E. MW: 29.9 kDa. Tags: C-terminal His-tag. a.a: full length. Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant Jellyfish Aequorea victoria green fluorescent protein, full length with C-terminal His-tag, MW 29.9 kDa, expressed in an E. coli expression system. GFP absorbs light at 395 and 470 nm, emits green fluorescence at 509 nm.
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