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gfp green fluorescent protein
Introduction to Fluorescent Proteins MicroscopyU.
This mutation is featured in the most popular variant of green fluorescent protein, termed enhanced GFP EGFP, which is commercially available in a wide range of vectors offered by BD Biosciences Clontech, one of the leaders in fluorescent protein technology.
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Green Fluorescent Protein Proteopedia, life in 3D.
11.0 11.1 11.2 11.3 11.4 11.5 6 Cubitt, AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien R. Understanding, improving, and using green fluorescent protein. Trends in Biochemical Sciences. Bublitz G, King BA, Boxer SG. Electronic structure of the chromophore in green fluorescent protein GFP.
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Green Fluorescent Protein GFP Arbor Assays Arbor Assays.
Final high resolution gel filtration purified. Green fluorescent protein GFP, a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light emission peak 509 nm when excited by blue light excitation peak 395 nm. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
GFP Green fluorescent protein Aequorea victoria Jellyfish GFP gene protein.
FACS-optimized" mutants of the green fluorescent protein GFP" Cormack B.P., Gene 17333-381996: PubMed Europe PMC Abstract. Cited for: NUCLEOTIDE SEQUENCE GENOMIC DNA, BIOTECHNOLOGY, MUTAGENESIS OF PHE-64; SER-65; VAL-68 AND SER-72. Understanding, improving and using green fluorescent proteins" Cubitt A.B., 20448-4551995: PubMed Europe PMC Abstract. Cited for: MUTAGENESIS OF TYR-66, BIOTECHNOLOGY. The" green fluorescent protein" Tsien R.Y. 67509-5441998: PubMed Europe PMC Abstract. Cited for: REVIEW ON MUTAGENESIS, BIOTECHNOLOGY. A" molecular thermometer based on fluorescent protein blinking" Wong F.H.,
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THE GREEN FLUORESCENT PROTEIN Annual Review of Biochemistry.
PASSIVE APPLICATIONS OF GFP. GFP AS AN ACTIVE INDICATOR. OUTLOOK FOR FUTURE RESEARCH. In just three years, the green fluorescent protein GFP from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology.
PDB-101: Molecule of the Month: Green Fluorescent Protein GFP.
Notice how the glycine and the threonine have formed a new bond, creating an unusual five-membered ring. To explore this structure in more detail, click on the image for an interactive JSmol. Topics for Further Discussion. You can explore an annotation of GFP and its family relationships at Interpro in their feature The Green Fluorescent Protein.
Green Fluorescent Protein Facts.
While his discoveries led to a better understanding of bioluminescence and fluorescence, this wild-type green fluorescent protein GFP was too difficult to obtain to have much practical application. In 1994, GFP was cloned, making it available for use in laboratories around the world.
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Green fluorescent protein PDB: 4EUL, often called enhanced green fluorescent protein, is a widely used variant of the wild-type green fluorescent protein PDB: 1GFL. Wild-type green fluorescent protein, or wt-GFP, occurs naturally in the jellyfish Aequorea victoria. wt-GFP has a minor excitation peak at 475 nm and a major excitation peak at 395 nm.
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