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gfp excitation
Wild-type GFP GFPuv fluorescent proteins.
Wild-type GFP GFPuv fluorescent proteins. Living Colors GFP and GFPuv are bacterial expression vectors that carry the gene for either GFP wild-type Aequorea victoria green fluorescent protein or GFPuv a GFP variant optimized for maximal fluorescence when excited by UV light described by Crameri et al.
Carnegie Cell Imaging.
GFP and YFP can be at least partially discriminated on the basis of careful filter selection. However, both excitation and emission spectra are fairly close together, limiting the discrimination possible and requiring difficult compromises to be made between brightness and discrimination.
Introduction to Fluorescence Microscopy Protocol.
The gene for GFP was originally isolated from jellyfish and can be expressed, or produced, by cultured cells in response to specific triggers or as part of a specific cell type like the tumor cells shown glowing in this image.
The success of wt-GFP and EGFP as reporters in the field of cell biology led researchers to search for more fluorescent proteins with different excitation and emission spectra. Of the fluorescent proteins found, DsRed PDB: 1G7K from the sea anemone Discosoma was especially valuable because its red-shifted fluorescence was quite visually distinct from that of GFP. mCherry PDB: 2H5Q from Discosoma is an optimized mutant of DsRed that is monomeric and fluoresces brighter than its predecessor. mCherry also has a faster chromophore maturation rate, improved photostability, and improved ability to serve as an N-terminal fusion tag 6. When compared to EGFP, mCherry is similar in many respects. mCherry is a small protein with a molecular weight of 26722.19, Da and a pI of 5.62 2.
Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy BMC Biotechnology Full Text.
While YFP has been used as part of a FRET pair in vivo 17, GFP was chosen over YFP because YFP is not effectively excited by wavelengths below 900 nm 18, YFP and GFP excitation closely mirror each other and are difficult to separate, and a large number of already available cell lines and transgenic animals use GFP.
GFP Chroma Technology Corp.
49009 ET Cy5 Narrow Excitation. 49010 ET RB Phycoerythrin/mOrange/mKO. 49011 ET FITC/Alexa Fluor 488/Fluo3/Oregon Green. 49012 ET FITC/EGFP Longpass. 49013 ET TFP Teal FP. 49014 ET mKO/mOrange. 49015 ET AlexaFluor 633. 49016 ET Cy3/TRITC Longpass. 49017 ET mCherry/Texas Red Longpass. 49018 ET EGFP Longpass. 49019 ET Cy5 Longpass. 49020 ET Narrow Band EGFP to Minimize Autofluorescence. 49021 ET EBFP2/Coumarin/Attenuated DAPI. 49022 ET Cy5.5. 49023 ET AlexaFluor 514/ZsYellow/Eosin. 49024 ET Keima Red/DiA. 49025 ET DAPI/FluoroGold Longpass. 49026 ET Tetracycline. 49027 ET Brilliant Violet 421, Alexa Fluor 405. 49028 ET DAPI for 395nm light sources. 49029 ET EYFP Longpass. 49030 ET Indocyanine Green. 49031 ET Alexa Fluor 568, Cy3.5. 49032 ET Brilliant Violet 480. 49037 ET Li_Cor for IR dye 800. 49052 ET ECFP/EYFP FRET. 49053 ET DAPI for 355-405nm LEDs. 49054 ET GFP for 455-465nm LEDs.
Green Fluorescent Protein: Properties, Applications and Protocols Google Livres.
Acad Aequorea GFP Aequorea victoria aequorin amino acid analysis Anthozoa assays bacteria Biochem Biochemistry bioluminescence Biotechnol Caenorhabditis elegans Cell Biol cellular Chale chromophore chromosome cloned Cnidaria color confocal coral Cormier detection dimer domains Drosophila DsRed dynamics efcient elegans embryos emission energy transfer excitation expressing GFP lter rst ow Fluorescent uorophore FRET function fusion protein gene expression genetic GFP expression GFP uorescence GFP fusion proteins GFP-like proteins green uorescent protein Heim identied imaging interactions jellysh living cells localization luciferase luciferin Lukyanov luminescence mammalian cells marker Matz membrane microscopy microtubules molecular molecules Morin mutants Natl neurons nuclear peptide photobleaching plasmid Prasher Proc quantum yield RCFPs receptor recombinant red uorescent protein Renilla luciferase residues screening sequence Shimomura signal specic spectral studies subcellular target tion tissue transcription transfected transgenic Tsien vectors visualized vivo Ward wavelengths wild-type GFP yeast zebrash.
Two-Photon Activation and Excitation Properties of PA-GFP in the 720920-nm Region ScienceDirect. ScienceDirect.
This report covers the two-photon activation and excitation properties of the PA-GFP, a photoactivatable variant of the Aequorea victoria green fluorescent protein in the spectral region from 720 to 920 nm. It is known from this special form of the molecule that it has an increased level of fluorescence emission when excited at 488 nm after irradiation at 413 nm, under single-photon excitation conditions.
A new twist in the photophysics of the GFP chromophore: a volume-conserving molecular torsion couple Chemical Science RSC Publishing.
The simple structure of the chromophore of the green fluorescent protein GFP, a phenol and an imidazolone ring linked by a methyne bridge, supports an exceptionally diverse range of excited state phenomena. Here we describe experimentally and theoretically the photochemistry of a novel sterically crowded nonplanar derivative of the GFP chromophore.

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